Microbes for biofuel: a cleaner way to unlock their energy

October 6, 2010

Algae and photosynthetic bacteria hold a hidden treasure – fat molecules known as lipids – which can be converted to renewable biofuels. Such microorganisms offer an attractive alternative to the unsustainable use of petroleum-based fossil fuels, as well as biofuel sources requiring arable cropland.

Cyanobacteria are capable of producing around 15,000 gallons of biofuel per acre – roughly 100 times that of plant or forest products including corn or switchgrass – and require only simple nutrients, sunlight and CO2 for growth. Download Full Image

But prying out the cellular ingredients needed for biofuels has so far come at a steep price, both economically and environmentally. Chemicals traditionally used in the process are extremely toxic.

Graduate researcher Jie Sheng and his colleagues at Arizona State University’s Biodesign Institute, have been exploring new methods for performing lipid extraction by less harmful means. Under the guidance of Bruce Rittmann, director of Biodesign’s Center for Environmental Biotechnology, the team successfully tested several formulas that recover lipid with high efficiency. The group’s results appear in the current issue of Bioresource Technology.

The two best candidates for photosynthetic biofuel production – algae and cyanobacteria – may be readily refined to produce a range of green gasolines, diesels, and other biofuels. But as Sheng notes, cyanobacteria offer several crucial advantages as a lipid source. “Cyanobacteria, particularly the strain we use, (known as Synechocystis) are very simple and have been fully sequenced genetically, so that we can easily modify them.”  Such genetic re-tooling would allow the quantity and quality of lipid production for biofuel to be optimized.

Further, unlike algae, which must be subjected to conditions of stress to maximize their lipid output, cyanobacteria are most successfully cultured under conditions of optimal growth, so that high-density lipid production is paired with a high rate of biomass production. “When the cell is provided with happy conditions for growth, we are able to get much more lipid out,” Sheng says.

But gathering the valuable lipids from cyanobacteria first requires the disruption of a tough, protective membrane. A half-century ago, Jordi Folch, a pioneering neurochemist, developed a method that remains the gold standard for isolating lipids from cells. The Folch method, as it is commonly known, has also been used by researchers to extract lipid from algae and cyanobacteria.

The technique involves the use of methanol and chloroform, which eat away and dissolve the lipids in a cell’s protective membrane, so that lipids may be harvested. For biofuel production, the Folch method is not practical, as large quantities of chloroform would wreak havoc on the environment and human health. (Chloroform, once a popular anesthetic, is categorized as a B2 chemical by the U.S. EPA – possibly carcinogenetic.)

Nevertheless, breaking down the durable thylakoid membrane of Synechocystis to get at the valuable lipids is not an easy task. As Sheng explains, alternate, less toxic chemicals have been used with success to extract lipid from algae, including ethanol, isopropanol, butanol, methyl tert-butyl ether (MTBE), acetic acid esters, hexane, and various combinations of these, but their viability for use with cyanobacteria was uncertain. Sheng’s team wanted to test such chloroform-free methods, to see if they could be used to extract lipids from Synechocystis.

In addition to the challenge of penetrating the more robust cyanobacterial cell membrane, Sheng notes that the lipids found in cyanobacteria are distinct from the lipids found in algae, vegetable and animal tissue, and the extraction methods may not work.

In a series of experiments, the team first demonstrated that the Folch method, as well as a closely related technique (Bligh & Dyer), were the most efficient means of lipid extraction for Synechocystis. Electron microscopy imaging showed effective penetration of the cell membrane and the ability to extract cyanobacterial lipids with high specificity.

The results closely matched the predictions the group had made through molecular modeling of the process. In contrast, ethanol, isopropanol, butanol, acetic ester, hexane, and combinations of these chemicals were significantly less effective in recovering lipid and were less specific, also recovering more impurities.

Intriguingly, the combination of methanol and MTBE showed high efficiency in cell penetration and lipid recovery, roughly comparable to the Folch and Bligh & Dyer methods. The group believes the combination of methanol and MTBE, which allows for the reduction of methanol and eliminates chloroform, may lower the toxicity  and environmental impact of the extraction process.

The research was carried out through the collaborative efforts of biologists and engineers, and continuing work in conjunction with other teams at the Biodesign Institute will explore mutant strains of Synechosystis boasting much higher lipid yields than the wild variety used in these experiments.

Further studies, sponsored by Department of Energy, involve genetically modifying Synechosystis so that the portion of the lipid refined into biofuel – the fatty acids – may be directly secreted through the cell wall. These and other ongoing efforts are helping to advance biofuel production from benchtop to eventual commercialization.

Written by Richard Harth
Biodesign Institute Science Writer
">mailto:richard.harth@asu.edu"> richard.harth@asu.edu

Britt Lewis

Communications Specialist, ASU Library

411 Gallery festivities celebrate community

October 6, 2010

The ASU Downtown Phoenix campus will open its doors to the community for the “Visions and Pathways toward Social Justice and Human Rights” 411 Gallery exhibition from 6 p.m. to 8 p.m., Nov. 5. The event will take place at the University Center at 411 N. Central Avenue in Phoenix.

The exhibition will showcase local nonprofits that are engaging in social justice and human rights issues such as health, education, labor, human, community and economic development, refugee rights, gender, and leisure. The exhibition shares stories, increases nonprofit mission visibility, and provides opportunities to connect and take action. Download Full Image

The “For Our Eyes” art exhibition shares timely stories through the artwork of community and university organizations, artists and members. More than 20 nonprofit groups are taking part, including the Cultural Arts Coalition, PSA Art Awakenings, GLSEN/Anti-Defamation League, Worker’s Rights Center, Advocates for Latin@ Arts and Culture, Release the Fear, Tumbleweed, Lodestar Day Resource Center, Big Brothers Big Sisters, Student s for Social Justice in Palestine, International Rescue Committee, the Lincoln Family YMCA and many more.  

“For Our Eyes” is grounded in ASU’s College of Public Programs’ commitment to the social and economic advancement of the diverse communities in the metropolitan region. The College is a center of intellectual and cultural engagement, within the vibrant urban core of the City of Phoenix, with knowledge at its foundation. From the design of the campus throughout the heart of downtown, to the proximity of hundreds of community organizations, “For Our Eyes” Exhibitions offers an unparalleled opportunity to make a difference by directly connecting with the people it serves. The “411 Gallery” is free and open during ASU building hours.

The exhibit is part of “First Friday” and will be conveniently located across the street from the Civic Space Park Collaboration’s “First Friday” November event. The Civic Space Park will be filled with performances, live art demonstrations, and Dia de los Muertos festivities.

The 411 Gallery promotes the use of Artlink shuttle stops. There is a stop located at the Artlink A.E. England Gallery in the Civic Space Park.

For information about the exhibit, contact Malissa Geer at malissa.geer">mailto:malissa.geer@asu.edu">malissa.geer@asu.edu.

Dana Berchman, dana.berchman">mailto:dana.berchman@asu.edu">dana.berchman@asu.edu
ASU College of Public Programs