Imaging at the speed of life

The European XFEL marks a new age of protein moviemaking that enables enzymes involved in disease to be observed in real time

November 18, 2019

To study the swiftness of biology — the protein chemistry behind every life function — scientists need to see molecules changing and interacting in unimaginably rapid time increments: trillionths of a second or shorter.

Imaging equipment with that kind of speed was finally tested last year at the European X-ray Free-Electron Laser, or EuXFEL. illustration of xfel The EuXFEL produces intense X-rays in extremely short pulses at a rate of one megahertz — a million pulses a second. The rays are aimed at crystals containing proteins, in a method called X-ray crystallography. When a crystal is hit by the X-ray pulse, it diffracts the beam, scattering in a certain pattern that reveals where the atoms are and producing a “snapshot.” Copyright: European XFEL / Blue Clay Studios Download Full Image

Now, Arizona State University researchers join a team of physicists from the University of Wisconsin-Milwaukee and their international collaborators to present EuXFEL’s first molecular movie, or “mapping,” of the ultrafast movement of proteins.

With this capability, scientists can watch how proteins do their jobs properly — or how their shape-changing goes awry, causing disease.

“Creating maps of a protein’s physical functioning opens the door to answering much bigger biological questions,” said Marius Schmidt, a professor of physics at the University of Wisconsin-Milwaukee (UWM) who designed the experiment and is lead author of the new study. “You could say that the EuXFEL can now be looked on as a tool that helps to save lives.”

Petra Fromme, who directs the Biodesign Center for Applied Structural Discovery, is very excited about the impact of the study.

"All processes in nature are highly dynamic, but most structures determined to date show only a static picture of a molecule. Time-resolved serial femtosecond crystallography at the European XFEL allows us to 'see,' in atomic detail, the dynamics of this photosensor protein in real time," Fromme said. 

Fromme helped oversee ASU’s contribution to the multifaceted EuXFEL project. In addition to her research at the Biodesign Institute, she is a Regents Professor in ASU’s School of Molecular Sciences.

The group’s findings signal the arrival of a new age of protein research enabling enzymes involved in disease to be observed in real time for meaningful durations in unprecedented clarity. The paper is published online in the journal Nature Methods.

Blaze of light

The EuXFEL produces intense X-rays in extremely short pulses at a megahertz rate — a million pulses a second. The rays are aimed at crystals containing proteins, in a method called X-ray crystallography. When a crystal is hit by the X-ray pulse, it diffracts the beam, scattering in a certain pattern that reveals where the atoms are and producing a “snapshot.”

The rapid-fire X-ray pulses produce 2D snapshots of each pattern from hundreds of thousands of angles where the beam lands on the crystal. Those are mathematically reconstructed into moving 3D images that show changes in the arrangement of atoms over time.

The European XFEL, which opened last year, has taken this atom mapping to a new level. Extremely powerful bursts contain X-ray pulses at a quadrillionth of a second, in “bursts” that occur at 100 millisecond intervals.

The new experiments began with a flash of blue, visible light that induced a chemical reaction inside the protein crystal, followed immediately by a burst of intense X-rays in megahertz pulses that produce the “snapshots.”

Initial research was staged in 2014 at the U.S. Department of Energy’s SLAC National Accelerator Laboratory in California, where Fromme, Schmidt and their colleagues were able to document atomic changes in their protein samples for the first time at an XFEL.

Subsequently, in 2016, they were able to map the rearrangement of atoms in the range of time proteins take to change their shapes — quadrillionths of a second (femtoseconds) up to 3 trillionths of a second (picoseconds). In a picosecond, which is a trillionth of a second, light travels the length of the period at the end of this sentence.

Previous time-resolved crystallography on their photoreactive protein had already been completed using other X-ray sources capable of imaging time scales larger than 100 picoseconds, leaving a gap of uncharted time between 3 and 100 picoseconds that the scientists were able to fill using the EuXFEL.

The exceptional brightness of the laser and the megahertz X-ray pulse rate allows much quicker data gathering with greater resolution over longer time frames.   

Europe’s X-ray super laser

The EuXFEL is an extreme device. At 3 kilometers long, spanning the distance between the German federal states of Hamburg and Schleswig-Holstein, it is the largest XFEL in the world. Superconductive technology is used to accelerate high-energy electrons, which generates the X-rays.

For their experiments, the scientists used the instrument for Single Particles, Clusters, and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) at EuXFEL. The instrument is designed to use the extremely short X-ray pulses for imaging and crystallography experiments in order to explore the structure and dynamics of small biological samples.

The team at the SPB/SFX instrument are able to adjust the parameters for each experiment depending on the protein being studied. These include changing the spot size of the optical light pulse to take into account the size of the individual protein crystals, and the time delay between optical and X-ray laser to accommodate the duration of the reaction being studied.

“The knowledge and expertise we have acquired with these experiments at the European XFEL will enable scientists to use the SPB/SFX instrument to produce molecular movies of their sample,” said Adrian Mancuso, European XFEL group leader at the SPB/SFX instrument where the study was done.

The new study extends recent research, promising important medical advances by means of enhanced crystallography with XFELs. Using this method, Fromme and fellow researchers have witnessed how multiple proteins work together, how enzymes responsible for antibiotic resistance disable a drug and how proteins change their shape in order to absorb light and enable sight.

The first author of the new study is doctoral student Suraj Pandey, who came to UWM from his native Nepal. Pandey’s role was to analyze the data and calculate the maps of structural change. Of the millions of X-ray pulses that XFELs deliver, the majority don’t hit a target at all. In fact, only 1% to 2% diffract off a protein crystal, while the remaining pulses produce “noise” that must be removed from the data.

Growing the specialized protein crystals required for the experiments was also challenging, and Pandey says that some of the invaluable specimens melted during their transit to Germany when they were held up at customs.

The ultimate success of the new experiments gave Pandey tremendous satisfaction.

“It’s a one-of-a-kind technology,” he said of the EuXFEL. “We pioneered the usage of European XFEL in seeing the movies of how proteins function. I’m just flying.”

Molecular cinema

Traditional structural biology methods use X-rays to produce snapshots of the 3D structure of molecules, including proteins. Although valuable, this information does not reveal details about the dynamics of biomolecular processes. If several snapshots can be taken in fast enough succession, however, these can be pasted together to make a so-called molecular movie.

The high repetition rate of the extremely short X-ray pulses produced by the European XFEL makes it now possible to collect large amounts of data to produce movies with more frames than ever before. An international group of scientists have now worked out how to make optimal use of the European XFEL’s very high X-ray repetition rate to make these molecular movies at the facility in order to reveal unprecedented details of our world.

Schmidt is also enthusiastic about the power of XFEL technology to revolutionize scientist’s understanding of dynamical properties that had long remained out of reach for investigation.

“Imagine a movie of a downhill ski race made up of only three frames. This will tell you something about how the race began and finished, and give you an image of the skier as well as a glimpse of the landscape somewhere on the mountain, but not much else. A movie containing many more frames, however, would follow the race in detail and would be much more informative,” he said. “Now that we understand how to harness the European XFEL X-ray pulses to collect large amounts of data to produce detailed molecular movies, we are very excited to see what unimagined insights are now possible.”

The work was carried out with BioXFEL collaborators from Arizona State University and the Deutsches Elektronen-Synchrotron (DESY) and beamline research specialists at the EuXFEL.

ASU coauthors on the paper include members of the Biodesign Center for Applied Structural Discovery and ASU’s School of Molecular Sciences including Jorvani Cruz Villarreal, Austin Echelmeier, Diandra Doppler, Raimund Fromme, Daihyun Kim, Alexandra Ros, Petra Fromme as well as Stella Lisova from ASU’s Department of Physics.

Adapted from story by Laura Otto, senior science writer/media relations, University Communications, UW-Milwaukee
Richard Harth

Science writer, Biodesign Institute at ASU


Veterans in Arizona more than twice as likely to die by suicide as nonveterans, ASU researchers find

November 18, 2019

Veterans in Arizona are at more than twice the risk of the rest of the population of dying by suicide, according to new information from Arizona State University’s Center for Violence Prevention and Community Safety (CVPCS).

Researchers at the CVPCS, based at ASU’s Watts College of Public Service and Community Solutions, found that 53.2 veterans per 100,000 population in Arizona killed themselves between 2015 and 2017, compared with a rate of 21.1 per 100,000 for nonveterans, said Professor Charles Katz, the Watts Family Director of the center. Download Full Image

The center released its Arizona Violent Death Reporting System (AZ-VDRS) report, "Suicides Involving Veterans," this month. In compiling their results, researchers examined 3,601 suicides that occurred in Arizona between 2015 and 2017. More findings:

  • Female veterans’ suicides occurred at about three times the rate of nonveteran females (28.9 per 100,000 population for female veterans versus 10.7 per 100,000 population among women who were not veterans).
  • More than 1 in 3 veteran suicide victims reported a physical health problem, compared with less than 1 in 4 among nonveteran suicide victims.
  • Veterans were far more likely to have used a firearm than nonveterans in killing themselves (80% versus 53.4%).

Younger veterans ages 18-34 are four times more likely to die by suicide than nonveterans of the same age group, according to the study.

Among Arizona counties, Mohave County in northwest Arizona leads the state in the rate of veteran suicides, with 79.3 veteran suicides per 100,000 population. Graham County in southeast Arizona had the lowest rate, logging 14.7 veteran suicides per 100,000 population. The statewide number is 50.4.

“If we as a state and a nation are serious about preventing suicide among our veterans, increased support for mental health screening and treatment after diagnosis is needed urgently,” the report’s implications and recommendations sections said. “Critically, we owe veteran men and women the highest standard of care and a rapid, effective response when they have disclosed suicidal thoughts and intentions or have survived actual attempts. The goal should be nothing less than the restoration of their potential for quality of life.”

Katz agreed.

“Our findings should give pause to all of us who support our troops, especially as we honor veterans this month,” he said. “Many of these suicides are the result of the physical and mental problems they have experienced. If you are close to a veteran, talk to them, communicate with them. Most of all, sympathize or empathize with the experiences they share with you, and encourage them to contact their local veterans center, VA medical center or a suicide prevention coordinator.”

The research for this report, like others the center conducts for the National Violent Death Reporting System (NVDRS) and the Centers for Disease Control and Prevention (CDC), has usefulness beyond compiling statistics, said David Choate, the CVPCS’ associate director.

“It is not research for the sake of research. Indeed, the mission of the CDC, the NVDRS and the Center for Violence Prevention and Community Safety is to see the AZ-VDRS data be put to use,” Choate said. “We work closely with law enforcement and public health partners, allowing both nongovernmental organizations and governmental policymakers much-needed information necessary for data-driven decision making in response to important issues surrounding homicides and suicides in Arizona.”

Mark J. Scarp

Media Relations Officer, Watts College of Public Service and Community Solutions