image title

A deep dive through time to study changing ecosystems

October 12, 2021

ASU to store samples from ongoing 30-year collection observing long-term effects on range of organisms

In 1999, ecologist J. Michael Fay spent 465 days hiking 2,000 miles across the Congo Basin of Africa while surveying the ecological and environmental status of the region. His feat became known as the MegaTransect.

In ecology, a transect is a survey of the flora and fauna of a particular area. It’s like putting a place in a test tube.

A three-decade transect through time is being funded by the federal government, and Arizona State University is playing a part.

In Tempe’s industrial area south of Broadway Road sits the ASU Natural History Collection, a huge facility of labs, storerooms, and nine collections of plants, mammals, fish, birds, reptiles, insects and other types of flora and fauna.

But slowly swelling its ranks is a special collection. Instead of most biological samples, where the intent is to learn more about the individual organism, this is a transect through time; a three-decade attempt to better understand how U.S. ecosystems are changing.

It’s called the NEON Biorepository.

The National Science Foundation's National Ecological Observatory Network, or NEON, is a research project with 81 locations across the U.S., including Hawaii, Puerto Rico and Alaska. The facilities collect long-term, open-access ecological data to observe changes to U.S. ecosystems.

The point is to observe long-term changes to ecosystems, like the abundance and locality of a species. Studying multiple samples simultaneously demonstrates the environmental changes' effects on different kinds of organisms. By studying 70 types of samples, researchers hope to unravel the complexity of the ecological responses to environmental change.

The 81 field sites across the country are divided among 20 ecoclimatic domains that represent regions of distinct landforms, vegetation, climate and ecosystem dynamics. For instance, Arizona is divided into two areas that could not be more different: the Sonoran Desert in the south and the Colorado Plateau up north.

There are two collection spots in Arizona — one terrestrial and one aquatic: Sycamore Creek northeast of the Valley, a popular camping and swimming spot for decades, and the Santa Rita Experimental Range and Wildlife Area south of Tucson.

The NEON Biorepository at ASU is the primary host of the samples from across the country. Project manager Kelsey Yule shared some numbers: 70 types of samples collected, with more than 100,000 samples coming in the door each year. By the end of the project, 3 million samples will be housed here.

Eight full-time NEON staffers process the samples and store them as they come in.

The collection started receiving samples in late 2018. Most of the samples are pretty small — no one is bringing in a mule deer every year. 

“Not a lot of vertebrates get collected,” Yule said.

There are samples of soil, blood, feces and DNA. Many of them go into four liquid nitrogen freezers and 19 mechanical freezers. It’s minus 196 degrees Celsius (minus 321 degrees Fahrenheit) in the nitrogen freezers and minus 80 C (minus 112 F) in the mechanical ones. By comparison, your home freezer is minus 18 C (about zero F). For context, the coldest temperature ever recorded at ground level was minus 89 C (minus 128 F) in July 1983 by researchers at Russia's Vostok Station on the East Antarctic Plateau.

Researchers are already using the material at NEON Biorepository. There are 22 projects ongoing, with samples out on loan. The topics are varied, from using machine learning to study changes in ground-dwelling invertebrate communities across the continent, to understanding nutrient composition and microorganism communities in airborne dust — projects at other universities that the ASU team is helping to facilitate.

"Every specimen or sample that we get from NEON has an online record that anyone can look at what all of the specimens we have here are," Yule said. "They're doing this project in order to allow researchers this really big standardized set of data over the next 30 years, so they can look at how they're changing over space and organisms and in Arizona."

To learn more, visit

Top photo: NEON cryo collections manager Azhar Husain shows soil samples being kept in the NEON Biorepository at ASU's Alameda Natural History Center in Tempe, Arizona, on Sept. 13. Photo by Deanna Dent/ASU News

Scott Seckel

Reporter , ASU News

image title

Buggin' around in southern Arizona

September 2, 2021

After a strong monsoon, ASU researchers are able to study a wealth of insect specimens

Editor's note: Arizona State University photographer and videographer Deanna Dent recently joined researchers from the Biodiversity Knowledge Integration Center on a trip to southern Arizona, where they collected insects after a strong monsoon. Here's what she learned.

This year may well be one of southern Arizona’s wettest monsoons in recorded history, and that has translated into vibrant plant growth and an increase in insect and animal populations after years of extreme drought.

A view of the grasslands

The photo above shows a large pond in the grasslands near the U.S.-Mexico border, east of Nogales, Arizona, and is exactly what researchers from BioKIC, or the Biodiversity Knowledge Integration Center, wanted to see. The researchers planned out a monsoon collection trip this past weekend, and while it's open to researchers, students and their families, they were kind enough to invite me with them as well. As an Arizona native it felt like a rare chance to go onto our public lands and learn about the plants, insects and animals of southern Arizona.

in the forest

When I first arrived at the Coronado National Forest campsite on Friday night, white sheets were being set up, glowing brightly in the darkness. These white sheets are clamped to a metal frame with a mercury vapor light at top, and a black light resting in the middle of the sheet. The lights were connected to a generator and run until midnight, attracting all types of insects from the dark. Between dinner and breaks, chatting folks examined the sheets to see what had been attracted, commenting at the variety and beauty of the insects. Researchers opted between cupping the small insects in their hands and placing them in vials or jars, or using the aspirator, a kind of vaccuum that pulls in small insects with a filter protecting the operators. These methods work well, but what struck me as interesting was that experts don't know why insects are attracted to the lights. They have theories, but no one knows why they're drawn to bright lights.

students collect under the lights


collecting insects at night

moths on sheet

The students, researchers and community members all spend time together, creating a strong sense of community interest. I'm perfectly welcome to ask a series of silly questions to experts in their field about how many insect species exist in the world or why they collect this beetle but not that one. Or why are moths dusty? And thankfully there are no unwelcome questions.

I ask, "Why this particular area of the Patagonia mountains?"

"This kind of highlands region, and to the Santa Rita mountains north of us, really contained a lot of interesting records from jaguars to a lot of reptiles and amphibians that just get into the U.S. from Mexico and extend their range into here," said Andrew Johnston, invertebrates collections manager and darkling beetle researcher. "So there were a lot of potentially interesting things for us to find in the less documented mountain range."

As the night continues, we find the Arizona state reptile, an Arizona ridge-nosed rattlesnake, curled at the base of a tree just a few feet from one campsite. We walk the small creek looking for frogs and toads, which you can hear but not really see. One camper shares the cicada they found preparing to molt, while botanist Elizabeth Makings shares a desert stink beetle and a dragonfly rests on the white sheet.





I also learned that although experts are very familiar with all the mammal species on Earth, there's a huge question mark on insect species. 

"It’s likely that we’ve not described even half of the diversity of insects that currently exist and alive in the world today," Johnston said.

A lack of knowledge of the insect life in an area adds to a lack of future understanding about our environment and ecological systems, as climate change affects our state over time. 

The generators go out at midnight and folks head to tents for the evening. As the sun rises over the mountains, green stretches all across the mountains and grasslands below, a shocking green that almost seems out of place in Arizona nowadays.



In the morning, Makings and researcher Ed Gilbert head up a stream that runs through the campsite with three ASU undergraduate students — Ethan Wright, Marcus Reid and Mary Haddad. Makings spends time walking with the students as they explore the grasses, plants, insects and the environment.

"I love to do fieldwork; I mean, why wouldn't you get buggy and hot and sweaty," said Makings, who helps organize the trips. "It's all about the camaraderie and, you know, interacting with scientists that are ... amazing in their field, and I just enjoy learning from everybody."

Gilbert's son, Aven Klecker, manages to catch a fencepost lizard with his lizard lasso. While holding the lizard in his hand, he flips it upside down and gently rubs its belly, which puts it into a still state. He shows the trick to Haddad, who holds the lizard until he jumps up and runs aways.



person examining roots of plant

As we concluded our walk, another set of researchers jumped in four-wheel-drive vehicles to explore the mountaintop areas reachable only by some rough forest roads. As we drive through some of the most scenic areas, we look for areas of interest and finally stop at a high point with a depression that has filled with water, creating a kind of pond in the center.

Researchers head out into the water with their nets to catch dragonflies or photograph botanical species. With every interesting find, everyone rushes over to take a look before the insects are released. School of Sustainability researcher Rick Overson spends time photographing the different insects with his 100mm macro lens, and environmental and zoological collections manager Laura Steger uses her net to collect dragonflies before we finally head back to camp. After a quick lunch, another group of researchers heads down to the nearly chest-height grassland areas near the U.S.-Mexico border. 

green fields

mountain top


Just near Lochiel, Arizona, we pull off on a road and head to a grassy area, after being rained out of the road to Scotia Canyon in the Huachuca Mountains. We can see the monsoon storm clouds all around but somehow don't receive a hint of rain. The grasses give way to a small pond where we find a frog and a western patch-nosed snake, which apparently is a county over from where it is described as having range; this is noted. We patch a punctured tire and someone passes around Trader Joe's cookies as we decide to head up to reach the campsite before dark. Folks are trying to bet whether the rains hit our campsite and if their sleeping bags will be soaked. 

US-Mexico fence



We reach the camp to find that it has not only rained but hailed, and while those who stayed behind tried their best to protect the area, everything is wet and damp. Unfortunately this means most insects won't come out to the white sheet at all, so we sit down and eat a spaghetti dinner together. Folks share photos from the day's finds. We hear jokes and stories about past trips and almost impassible roads, along with a limerick or two before everyone heads to bed.

The next morning everyone sits down for breakfast and a final touch-base as the camp breaks down. I interview Johnston and Makings and record sound of the stream, which is running with water this morning, and plan out my route back home through Patagonia to Tucson and back to Phoenix. Makings is leaving with over 150 grass specimens — a wealth of samples after years of drought, which may take weeks to process back at the Alameda Center.

Johnston shows me the tools of the trade, including forceps, an aspirator, nets and archival vials — though takeout containers can work temporarily as well. He says sometimes he saves samples in his freezer until he's ready to process, and it makes me wonder if that ever surprises anyone. It's a bright beautiful morning as we all head back to regular life, and I'm glad I spent this weekend out in the wilderness with such an amazing group of experts. 

breakfast at camp

tools of the trade